Detection of Macroprolactin Causing Hyperprolactinemia in Commercial Assays for Prolactin
نویسندگان
چکیده
منابع مشابه
Detection of macroprolactin causing hyperprolactinemia in commercial assays for prolactin.
one at the concentration of 7 pg/mg. This result was not challenged by the athlete. Nandrolone has also been identified by this laboratory in the hair of bodybuilders, in concentrations of 1–7 pg/mg (8 ). NA and noretiocholanolone were not detected in any hair. The sensitive, specific, and reproducible method developed appears to be suitable for the detection and quantification of 19-norsteroid...
متن کاملIn hyperprolactinemia, testing for macroprolactin is essential.
Autonomous secretion of prolactin (PRL) by a pituitary prolactinoma is a relatively common endocrine disorder characterized by increased serum concentrations of PRL and symptoms of menstrual irregularity, infertility, and galactorrhea in women and impotence and lack of libido in men (1 ). These clinical symptoms are common, and measurement of serum PRL is a key investigation used to identify th...
متن کاملMacroprolactin reactivities in prolactin assays: an issue for clinical laboratories and equipment manufacturers.
was performed in 150 mL of a 1 g/L silver nitrate solution for 10 min at room temperature with gentle shaking. The gel was rinsed twice with distilled water to remove excess silver solution and was developed in a solution containing, per liter, 15g of NaOH, 0.1 g of NaBH4, and 0.48 g of formaldehyde. The gel was first washed in 150 mL of this solution (for ;30 s) to precipitate the excess silve...
متن کاملMacroprolactin; A Frequent Cause of Misdiagnosed Hyperprolactinemia in Clinical Practice
INTRODUCTION Macroprolactin is a significant cause of misdiagnosis, unnecessary investigation, and inappropriate treatment in patients with hyperprolactinemia. Its frequency has not been clearly established due to technical difficulties in identifying it. Most laboratories and clinicians are unaware of macroprolactin interferences in prolactin assays. MATERIALS AND METHODS A comprehensive lit...
متن کاملHyperprolactinemia: new assay more specific for the monomeric form does not eliminate screening for macroprolactin with polyethylene glycol precipitation.
Prolactin presents important size heterogeneity in terms of circulating molecular forms. Besides the monomer (23kDa), which predominates in healthy subjects as well as in patients with prolactinoma, high molecular forms are also present in low concentrations (1). These high molecular forms include 50 kDa dimers (big prolactin) and also forms with even larger molecular weights (MW>150 kDa), know...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2000
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/46.12.2022